Proteome analysis of Chinese miniswine bone marrow mesenchymal stem cells

The proteome of Chinese miniswine bone marrow mesenchymal stem cells (MSCs) was investigated in this study. Proteins extracted from MSCs were separated by two-dimensional gel electrophoresis (2DE), and about 1600 protein spots were detected using the silver nitrate staining method. 48 proteins were digested by trypsin for mass spectrometry analysis. 37 proteins were successfully identified by peptide mass fingerprinting (PMF). The biological functions of these identified proteins were involved in different cell processes. Proteomics was successfully applied for the protein expression analysis of Chinese miniswine bone marrow MSCs and some useful data were provided for the future study of MSCs.