Separation of Amino Acids by In-situ Polymerized Anion Exchange Micro-monolithic Column on Micro-fluidic Chip

Alkali quaternary ammonium anion exchange micro-monolithic column based on monomer of [2-(methacryloyloxy)ethyl] trimethylammonium chloride (META) was polymerized in-situ. The analysis of amino acids-H2O2-Luminol chemiluminescence system was conducted with the application of the micro-monolithic column combined with micro-fluidic chip. According to the different isoelectric point (pI) of amino acids, separations were performed using the micro-monolithic column. The separations of amino acid samples, including phenylalanine and leucine/histidine, were carried out on the constructed microchip. The results showed that complete separations of samples were realized. Several factors affecting separation of amino acids, such as buffer pH, pH and flow rate of mobile phase were investigated, by which the resolution of phenylalanine and arginine detected was 1.6. The results also demonstrated feasibility of integrating the micro-monolithic column with micro-fluidic chip.