Analysis of the surface, secreted, and intracellular proteome of Propionibacterium acnes

• We quantify the secreted, cell wall, membrane, and cytosolic proteome of P. acnes.
• 531 proteins were identified and analyzed for localization and function.
• Several surface proteins were identified as potential vaccine candidates.

Propionibacterium acnes, plays an important role in acne vulgaris and other diseases. However, understanding of the exact mechanisms of P. acnes pathogenesis is limited. Few studies have investigated its proteome, which is essential for vaccine development. Here, we comprehensively investigate the proteome of P. acnes strain ATCC 6919, including secreted, cell wall, membrane, and cytosolic fractions in three types of growth media. A total of 531 proteins were quantified using an Orbitrap mass spectrometer and bioinformatically categorized for localization and function. Several, including PPA1939, a highly expressed surface and secreted protein, were identified as potential vaccine candidates.

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