Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure

• 2D RP/RP LC–MS/MS improves proteome and protein coverage relative to 1D analysis.
• Sulforaphane does not influence global protein profile in LNCaP prostate cancer cells.
• Global analysis identified the protein TRIAP1 in LNCaP cells.
• TRIAP1 influences LNCaP proliferation and is a potential therapeutic target.

The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC–MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC–MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.

Figure optionsDownload as PowerPoint slide