کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1184883 1492151 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibitory effect of skullcap (Scutellaria baicalensis) extract on ovalbumin permeation in vitro and in vivo
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Inhibitory effect of skullcap (Scutellaria baicalensis) extract on ovalbumin permeation in vitro and in vivo
چکیده انگلیسی

Scutellaria baicalensis Georgi (skullcap) has been widely used as a dietary ingredient. The purpose of this study was to reveal novel function of skullcap and its mechanism on allergen permeation in intestinal epithelial cells. Intestinal epithelial Caco-2 cell monolayers were used to evaluate the inhibitory effect of skullcap on ovalbumin (OVA) permeation by measuring transepithelial electrical resistance (TEER) and the quantity of permeated OVA. TEER increased and the OVA flux decreased in a dose-dependent manner through up-regulating tight junction-related proteins in cells incubated with increasing concentrations of skullcap extract. In the in vivo study, the amounts of OVA from orally ingested albumen reduced on administration of the skullcap extract. We also revealed for the first time that the active component of skullcap extract for inhibition of OVA permeation was baicalein. These findings demonstrated that skullcap extract might attenuate a food allergic response by inhibiting allergen permeation in vitro and in vivo.


► Skullcap extract inhibits ovalbumin permeation across intestinal epithelium.
► Skullcap extract enhances intestinal barrier function.
► Skullcap extract up-regulates mRNA expression of tight junction-related proteins.
► Skullcap extract could attenuate food allergy by inhibiting allergen permeation.
► Baicalein was the active component of skullcap extract.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 140, Issues 1–2, 1–15 September 2013, Pages 22–30
نویسندگان
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