کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1186021 963422 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improved sample treatment and chromatographic method for the determination of isoflavones in supplemented foods
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Improved sample treatment and chromatographic method for the determination of isoflavones in supplemented foods
چکیده انگلیسی

Isoflavones are a group of substances that belong to the family of phytoestrogens. These natural substances may offer several benefits to human health. One of the most important sources for human isoflavone intake is soy and soybean food derivatives. An improved sample treatment followed by a high performance liquid chromatographic method for the determination of isoflavones in supplemented milk and juices is proposed and compared to the AOAC official method. Detection limits found were between 0.2 and 0.3 mg L−1 for daidzein and genistein respectively. Quantification limits found were between 0.7 mg L−1 for daidzein to 1.0 mg L−1 for genistin, while inter and intra-day variability was under 10% in all cases. Recoveries for spiked samples were over 90% and under 110%. The method was validated by comparison with the AOAC method and by recovery assay methodology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 123, Issue 3, 1 December 2010, Pages 872–877
نویسندگان
, , , ,