کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1188113 | 963481 | 2012 | 6 صفحه PDF | دانلود رایگان |
Cecropin A gene was cloned into the expression vector pPIC9k and was successfully expressed in methylotrophic yeast, Pichia pastoris GS115. The yeast had effective antimicrobial activity on Geotrichum citri-aurantii spores by the thiazolyl blue (MTT) assay. There was no large growth difference between nontransformed strain GS115 and recombinant strain GS115/CEC in citrus fruits wounds. Yeast transformants could significantly inhibit growth of germinated G. citri-aurantii spores and inhibited decay development caused by G. citri-aurantii in citrus fruits compared to the yeast strain GS115/pPIC. This study demonstrates the potential of expression of an antifungal peptide in yeast for enhancing suppression of postharvest diseases and represents a new approach for the biological control of postharvest diseases.
► The study showed that cecropin A can inhibit G. citri-aurantii spores effectively.
► Recombinant strain GS115/CEC can express antifungal cecropin A successfully.
► GS115/CEC yeast is suitable for biocontrol tests on citrus fruit.
► GS115/CEC yeast can inhibit growth of G. citri-aurantiiin vitro and in vivo.
Journal: Food Chemistry - Volume 131, Issue 3, 1 April 2012, Pages 796–801