کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1190672 963537 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates during a simulated gastrointestinal digestion
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Changes in the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates during a simulated gastrointestinal digestion
چکیده انگلیسی

A two-stage in vitro digestion model system (a pepsin treatment for 2 h followed by a pancreatin treatment for 2 h, both at 37 °C) was used to simulate the process of human gastrointestinal (GI) digestion to determine the changes in antioxidant activities of loach peptide previously prepared by papain digestion. Results showed that the final GI digests contained 38.1% free amino acids, with short chain peptides (<500 Da) making up the rest of the biomass. Enzymatic breakdown of the GI digests increased their hydroxyl (12% increase), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) (5% increase) radical scavenging activity, the reducing power (77% increase) and the chelating ability of Cu2+ (12% increase), compared to the blank. The results showed that the digestion by gastrointestinal proteases can be used to produce antioxidant peptides, with the advantage that the peptides formed will resist physiological digestion in GI tract.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 120, Issue 3, 1 June 2010, Pages 810–816
نویسندگان
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