کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1191717 963580 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of pectinmethylesterase activity in presence of methanol during grape pomace storage
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Detection of pectinmethylesterase activity in presence of methanol during grape pomace storage
چکیده انگلیسی

Methanol is an unwanted component in the production of spirit beverages. Its presence in grape pomace stored until distillation greatly affects the composition of the final product, which requires redistillation, modifying the aromatic characteristics of the distillate. Consequently, reduction of methanol, by controlling its formation during pomace storage, can increase the quality of grappa. The aim of this work was to monitor pectinmethylesterase activity during grape pomace storage, in order to identify its presence related to methanol release. The enzyme activity was detected during various storage times by spectrophotometric and electrophoretic methods. Results show that yeasts do not contribute to PME production. Moreover, by using paranitrophenyl acetate, a specific substrate for esterase, also as PME substrate, we demonstrate the presence of several enzymes hydrolysing ester bonds.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 102, Issue 1, 2007, Pages 59–65
نویسندگان
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