کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1191751 | 963580 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dissociation of natural actomyosin from kuruma prawn muscle induced by pyrophosphate
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Effect of pyrophosphate (PP) on the dissociation and stability of natural actomyosin (NAM) from kuruma prawn muscle was studied in comparison with adenosine 5â²-triphosphate (ATP). In the presence of PP up to 5 mM, NAM exhibited lower Mg2+-ATPase activity (P < 0.05), while no marked change was observed in NAM treated with ATP at all concentrations tested (0.25-10 mM) (P > 0.05). Ca2+-ATPase activity of NAM treated with 5 mM PP decreased markedly when incubated at temperatures greater than 30 °C, suggesting lowered thermal stability of the liberated myosin molecule. Nevertheless, Ca2+-ATPase activity of ATP-treated NAM was similar to the control NAM. In the presence of 5-10 mM MgCl2, NAM treated with 5 mM PP underwent dissociation effectively, as evidenced by a greater decrease in Mg2+-ATPase activity as well as an increased band intensity of actin released. Therefore, addition of PP in combination with MgCl2 was more effective than was ATP in dissociating the actomyosin complex of prawn muscle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 102, Issue 1, 2007, Pages 295-301
Journal: Food Chemistry - Volume 102, Issue 1, 2007, Pages 295-301
نویسندگان
Soottawat Benjakul, Wonnop Visessanguan, Tanong Aewsiri, Munehiko Tanaka,