کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1195186 964292 2009 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis
چکیده انگلیسی

Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.

Graphical AbstractGas-phase disulfide bond scrambling during MALDI was observed for peptides, and a possible mechanism is proposed. The disulfide bond pattern in SEL24K was established unambiguously.Figure optionsDownload high-quality image (94 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 20, Issue 9, September 2009, Pages 1603–1616
نویسندگان
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