کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1195218 964297 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative–Qualitative Data Acquisition Using a Benchtop Orbitrap Mass Spectrometer
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantitative–Qualitative Data Acquisition Using a Benchtop Orbitrap Mass Spectrometer
چکیده انگلیسی

Current approaches to discovery-stage drug metabolism studies (pharmacokinetics, microsomal stability, etc.) typically use triple-quadrupole–based approaches for quantitative analysis. This necessitates the optimization of parameters such as Q1 and Q3 m/z values, collision energy, and interface voltages. These studies detect only the specified compound and information about other components, such as metabolites, is lost. The ability to perform full-scan acquisition for quantitative analysis would eliminate the need for compound optimization while enabling the detection of metabolites and other non-drug-related endogenous components. Such an instrument would have to provide sensitivity, selectivity, dynamic range, and scan speed suitable for discovery-stage quantitative studies. In this study, a prototype benchtop Orbitrap-based mass analyzer was used to collect both quantitative and qualitative data from human microsomal incubation samples as well as rat plasma from pharmacokinetic studies. Instrumental parameters such as scan speed, resolution, and mass accuracy are discussed in relation to the requirements for a quantitative–qualitative workflow. The ability to perform highly selective quantitative analysis while simultaneously characterizing metabolites from both in vitro and in vivo studies is discussed.

Graphical AbstractQuantitative and qualitative data acquisition for drug metabolism and pharmacokinetics–related drug discovery assays is achieved using high-resolution mass spectrometry on a benchtop Orbitrap mass spectrometer.Figure optionsDownload high-quality image (120 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 20, Issue 8, August 2009, Pages 1441–1450
نویسندگان
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