کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1195309 964317 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface-Induced Dissociation Shows Potential to Be More Informative Than Collision-Induced Dissociation for Structural Studies of Large Systems
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Surface-Induced Dissociation Shows Potential to Be More Informative Than Collision-Induced Dissociation for Structural Studies of Large Systems
چکیده انگلیسی

The ability to preserve noncovalent, macromolecular assemblies intact in the gas phase has paved the way for mass spectrometry to characterize ions of increasing size and become a powerful tool in the field of structural biology. Tandem mass spectrometry experiments have the potential to expand the capabilities of this technique through the gas-phase dissociation of macromolecular complexes, but collisions with small gas atoms currently provide very limited fragmentation. One alternative for dissociating large ions is to collide them into a surface, a more massive target. Here, we demonstrate the ability and benefit of fragmenting large protein complexes and inorganic salt clusters by surface-induced dissociation (SID), which provides more extensive fragmentation of these systems and shows promise as an activation method for ions of increasing size.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 19, Issue 7, July 2008, Pages 903–913
نویسندگان
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