کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1195344 964327 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigation of Ligand Interactions with Human RXRα by Hydrogen/Deuterium Exchange and Mass Spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Investigation of Ligand Interactions with Human RXRα by Hydrogen/Deuterium Exchange and Mass Spectrometry
چکیده انگلیسی

Several different agonists of the retinoic X receptor α (hRXRα) were examined for their effects on the amide H/D exchange kinetics of the homodimeric protein using mass spectrometry. Some agonists, LG 100268, SR11246, and DHA, bind such that slower deuterium exchange-in occurs compared with 9-cis-retinoic acid (9-cis-RA), whereas others, fenretinide and methoprenic acid, result in poorer protection during binding and hence faster exchange-in. Protection against H/D exchange by different agonists and the inhibition of H/D exchange kinetics relative to 9-cis-RA varies markedly in different regions of the protein. Agonists LG 100268, SR11246, and DHA generally inhibit faster exchange processes in the ligand binding regions of hRXRα than does the native ligand 9-cis-RA. In at least half of these regions, the level of protection by 9-cis-RA lags behind the agonists even after 60 min. Methoprenic acid did not significantly protect hRXRα against amide hydrogen exchange. An efficient method is described for comparing the effects of different agonists on the protein structure of the agonist-RXRα complex.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 17, Issue 11, November 2006, Pages 1510–1517
نویسندگان
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