کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1195646 | 964399 | 2010 | 6 صفحه PDF | دانلود رایگان |

Axial collision induced dissociation (CID) and high-pressure resonance CID were implemented and compared with normal low-pressure resonance CID in a miniature ion trap mass spectrometer to obtain more complete fragmentation spectra. Axial CID was realized simply by applying a potential to the discontinuous atmospheric pressure interface (DAPI) capillary without performing parent ion isolation before dissociation. High-pressure resonance CID employed a double-introduction pulse scan function, by means of which precursor ions isolated at low-pressure (<10−3 torr) were dissociated at high-pressure (0.1 torr–1 torr) with higher excitation energy, so that tandem MS of isolated precursor ions was achieved and extensive fragmentation was obtained. A simple peptide (Leu-enkephalin) and dye molecule (rhodamine B) ionized by ESI were used to investigate both methods and compare them with normal low-pressure resonance CID.
Graphical AbstractTwo new methods and one old method of CID in a miniature ion trap are compared.Figure optionsDownload high-quality image (159 K)Download as PowerPoint slide
Journal: Journal of the American Society for Mass Spectrometry - Volume 21, Issue 2, February 2010, Pages 209–214