Evaluation of Binding Selectivity of a Polyamide Probe to Single Base-Pair Different DNA in A·T-Rich Region by Electrospray Ionization Mass Spectrometry

In this study, electrospray ionization mass spectrometry (ESI-MS) was used for the evaluation of the binding selectivity of a polyamide probe to single-base pair different DNA in an A·T-rich region. In this procedure, ΔIr(dsn) was introduced as a parameter to compare the binding affinities of the polyamides with the duplex DNA. The results show that ESI-MS is a very useful tool for analysis of binding selectivity of a polyamide probe to single-base pair different DNA.