Solvation of Electrosprayed Ions in the Accumulation/Collision Hexapole of a Hybrid Q-FTMS

In an effort to spectroscopically determine the structures of solvated ions composed of nucleic acid bases and amino acids, methods for their gas-phase synthesis have been studied. Ions were electrosprayed and solvated in the accumulation cell of a hybrid Q-FTICR filled with methanol or water vapor at ∼10−2 bar. There were subsequently transferred to the FTICR cell at 10−10 mbar. Following their isolation in the FTICR, they can be investigated by studying their unimolecular blackbody infrared radiative dissociation (BIRD) or infrared multiple photon dissociation (IRMPD) spectroscopy. The IRMPD spectra for (Ade)2Li+ and (Ade)2Li(H2O)+ are reported and compared as well as BIRD rate constants for multiply solvated and metalated adenine ions.

Graphical AbstractElectrosprayed ions are resolvated by replacing the Ar-collision gas is in the hexapole accumulation cell with solvent for further study in an FTMS.Figure optionsDownload high-quality image (88 K)Download as PowerPoint slide