کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1195999 964441 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Liquid Matrix Deposition on Conductive Hydrophobic Surfaces for Tuning and Quantitation in UV-MALDI Mass Spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Liquid Matrix Deposition on Conductive Hydrophobic Surfaces for Tuning and Quantitation in UV-MALDI Mass Spectrometry
چکیده انگلیسی

With its highly fluctuating ion production matrix-assisted laser desorption/ionization (MALDI) poses many practical challenges for its application in mass spectrometry. Instrument tuning and quantitative ion abundance measurements using ion signal alone depend on a stable ion beam. Liquid MALDI matrices have been shown to be a promising alternative to the commonly used solid matrices. Their application in areas where a stable ion current is essential has been discussed but only limited data have been provided to demonstrate their practical use and advantages in the formation of stable MALDI ion beams. In this article we present experimental data showing high MALDI ion beam stability over more than two orders of magnitude at high analytical sensitivity (low femtomole amount prepared) for quantitative peptide abundance measurements and instrument tuning in a MALDI Q-TOF mass spectrometer. Samples were deposited on an inexpensive conductive hydrophobic surface and shrunk to droplets <10 nL in size. By using a sample droplet <10 nL it was possible to acquire data from a single irradiated spot for roughly 10,000 shots with little variation in ion signal intensity at a laser repetition rate of 5–20 Hz.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 18, Issue 4, April 2007, Pages 693–697
نویسندگان
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