Construction of a Versatile High Precision Ambient Ionization Source for Direct Analysis and Imaging

The design and construction of a high precision ambient ionization source matrix-assisted laser desorption electrospray ionization (MALDESI) are described in full detail, including a complete parts list. The computer controlled high precision motion control system and high repetition rate Explorer laser are demonstrated during MALDESI-FT-ICR analysis of peptides and proteins ranging from 1 to 17 kDa. The high stability ionization source platform described herein demonstrates both the advantages of the new MALDESI source and versatility for application to numerous desorption and ionization techniques.

Graphical AbstractWe present the development of a high precision ionization platform for direct analysis and high spatial resolution ambient imaging mass spectrometry.Figure optionsDownload high-quality image (197 K)Download as PowerPoint slide