کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1196112 964454 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of the Matrix on Analyte Fragmentation in Atmospheric Pressure MALDI
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Influence of the Matrix on Analyte Fragmentation in Atmospheric Pressure MALDI
چکیده انگلیسی

In this paper, we report the measurement of the degree of analyte fragmentation in AP-MALDI as a function of the matrix and of the laser fluence. The analytes include p-OCH3-benzylpyridinium, three peptides containing the sequence EEPP (which cleave very efficiently at the E-P site), and three deoxynucleosides (dA, dG, and dC), which lose the neutral sugar to give the protonated base. We found that the matrix hardness/softness was consistent when comparing the analytes, with a consensus ranking from hardest to softest: CHCA ≫ DHB > SA ≈ THAP > ATT > HPA. However, the exact ranking can be fluence-dependent, for example between ATT and HPA. Our goal here was to provide the scientific community with a detailed dataset that can be used to compare with theoretical predictions. We tried to correlate the consensus ranking with different matrix properties: sublimation or decomposition temperature (determined using thermogravimetry), analyte initial velocity, and matrix proton affinity. The best correlation was found with the matrix proton affinity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 17, Issue 7, July 2006, Pages 1005–1013
نویسندگان
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