کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1196190 964465 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
De Novo Peptide Sequencing Using Exhaustive Enumeration of Peptide Composition
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
De Novo Peptide Sequencing Using Exhaustive Enumeration of Peptide Composition
چکیده انگلیسی

We introduce the use of a peptide composition lookup table indexed by residual mass and number of amino acids for de novo sequencing of polypeptides. Polypeptides of 1600 Daltons (Da) or more can be sequenced effectively through exhaustive compositional analysis of MS/MS spectra obtained by unimolecular decomposition (without CID) in a MALDI TOF/TOF despite a fragment mass accuracy of 50 mDa. Peaks are referenced against the lookup table to obtain a complete profile of amino acid combinations, and combinations are assembled into series of increasing length. Concatenating the differences between successive entries in compositional series yields peptide sequences that can be scored and ranked according to signal intensity. While the current work involves measurements acquired on MALDI TOF-TOF, such general treatment of the data anticipates extension to other types of mass analyzers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society for Mass Spectrometry - Volume 17, Issue 8, August 2006, Pages 1041–1049
نویسندگان
, , ,