High Mass Selectivity for Top-Down Proteomics by Application of SWIFT Technology

Stored waveform inverse Fourier transform (SWIFT) technology has been implemented on a commercial Fourier transform ICR mass spectrometer. Complex ejection/isolation waveforms can be generated on an arbitrary waveform generator (AWG) and applied to the instrument by use of a high speed analog switch. High mass selectivity and subsequent electron capture dissociation (ECD) of the SWIFT isolated ions has been demonstrated with analysis of intact Bovine histone H4. A mass selectivity about 0.1 m/z unit for isolation of the 18+ charge state ions was achieved. Adaptation of SWIFT on the commercial instrument provides an enhanced capability for characterizing intact proteins by top-down analysis.

Graphical AbstractUltrahigh mass selectivity for isolation of intact proteins is achieved on a commercial LTQFT mass spectrometer by use of SWIFT technology.Figure optionsDownload high-quality image (71 K)Download as PowerPoint slide