A Study of Noncovalent Protein Complexes by Matrix-Assisted Laser Desorption/Ionization

A sample preparation method has been developed for detection of noncovalent protein–protein complexes by MALDI in this work. An aqueous solution of the matrix at pH 7 allows the reproducible detection of a protein dimer, a protein tetramer, and a heterodimer. The signals are stable under long irradiation and can be detected at wide ranges of concentrations and with different laser intensities.