کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1198643 1493476 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Specific targeted quantification combined with non-targeted metabolite profiling for quality evaluation of Gastrodia elata tubers from different geographical origins and cultivars
ترجمه فارسی عنوان
ارزیابی اختصاصی هدفمند همراه با مشخصات پروفایل متابولیسم غیر هدفمند برای ارزیابی کیفیت غده گاسترویدا الاطا از ریشه های مختلف جغرافیایی و ارقام مختلف
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A strategy integrating targeted and non-targeted analyses was proposed.
• A single standard to determine multi-components method was developed.
• Diverse GET samples were discriminated by non-targeted metabolite profiling.
• 147 marker metabolites were screened from 1194 aligned variables.
• Support vector machines was used to predict the geographical origins of GET.

Gastrodia elata tuber (GET) has been widely used as a famous herbal medicine in China and other East Asian countries. In this work, we developed a comprehensive strategy integrating targeted and non-targeted analyses for quality evaluation and discrimination of GET from different geographical origins and cultivars. Firstly, 43 batches of GET samples of five cultivars from three regions in China were efficiently quantified by a “single standard to determine multi-components” (SSDMC) method. Six marker compounds were simultaneously determined within 11 min using gastrodin as the internal standard. It showed that samples from different regions and cultivars could not be differentiated by the contents of six marker compounds. Secondly, a non-targeted metabolite profiling analysis was performed by ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). Samples from different geographical origins and cultivars were clearly discriminated by principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA). 147 discriminant ions contributing to the group separation were selected from 1194 aligned variables. Furthermore, based on the relative intensities of discriminant ions, support vector machines (SVM) was employed to predict the geographical origins of GET. The obtained SVM model showed excellent prediction performance with an average prediction accuracy of 100%. These results demonstrated that the UHPLC-QTOF/MS-based non-targeted metabolite profiling analysis, as a vital supplement to targeted analysis, can be used to discriminate the geographical origins and cultivars of GET.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1450, 10 June 2016, Pages 53–63
نویسندگان
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