کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1198702 1493484 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of pre- and post-column band broadening on the performance of high-speed chromatography columns under isocratic and gradient conditions
ترجمه فارسی عنوان
تاثیر انبساط باند پیش و پس از ستون بر عملکرد ستونهای کروماتوگرافی با سرعت بالا تحت شرایط ایزوکراتی و گرادیان
کلمات کلیدی
پراکندگی، رطوبت گرادیان، لوله اتصال مدل سازی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• The individual contributions to extra-column band broadening (ECBB) have been studied.
• The experimental results could be well fitted with existing ECBB models.
• The fitting was used to update the fitting parameters appearing in these models.
• We also quantified the strong sensitivity of gradient separations to post-column ECBB.

We report on the results of an experimental and theoretical study of the effect of the extra-column band broadening (ECBB) on the performance of narrow-bore columns filled with the smallest particles that are currently commercially available. Emphasis is on the difference between the effect of ECBB under gradient and isocratic conditions, as well as on the ability to model and predict the ECBB effects using well-established band broadening expressions available from the theory of chromatography. The fine details and assumptions that need to be taken into account when using these expressions are discussed. The experiments showed that, the steeper the gradient, the more pronounced the extra-column band broadening losses become. Whereas the pre-column band broadening can in both isocratic and gradient elution be avoided by playing on the possibilities to focus the analytes on top of the column (e.g. by using the POISe injection method when running isocratic separations), the post-column extra-column band broadening is inescapable in both cases. Inducing extra-column band broadening by changing the inner diameter of the post-column tubing from 65 to 250 μm, we found that all peaks in the chromatogram are strongly affected (around a factor of 1.9 increase in relative peak width) when running steep gradients, while usually only the first eluting peak was affected in the isocratic mode or when running shallow gradients (factor 1.6–1.8 increase in relative peak width for the first eluting analyte).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1442, 15 April 2016, Pages 73–82
نویسندگان
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