کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1198785 | 1493462 | 2016 | 9 صفحه PDF | دانلود رایگان |
• An evaluation of alternative solvents was performed in the field of lipid classes analysis.
• n-Heptane can be advantageously replaced by hexamethyldisiloxane.
• CPME, 2-MeTHF and isopentyl acetate have proved to be valuable mid-polarity.
• Comparable or even better separation than those obtained with conventional solvents were achieved.
An evaluation of solvents alternative to n-heptane (d-limonene and hexamethyldisiloxane) and chloroform (cyclopentyl methyl ether, 2-methyltetrahydrofuran and isopentyl acetate) was developed for lipid classes separation of non-polar cholesteryl ester to highly polar phospholipids by high-performance liquid chromatography on bare silica stationary phase and evaporative light-scattering detection. Screening of alternative solvents was used to estimate their compatibility with liquid chromatography and evaporative light-scattering detection and to evaluate their chromatographic selectivity. This work shows that n-heptane can be advantageously replaced by hexamethyldisiloxane. An increase of non-polar lipids retention is observed with hexamethyldisiloxane as weak solvent. Chloroform, which is largely used for lipid analysis, might be replaced efficaciously by cyclopentyl methyl ether, 2-methyltetrahydrofuran or isopentyl acetate. Aside from offering a different selectivity, the gradients composed by one or both alternative solvents gave efficient and comparable or even better separations than those obtained with conventional solvents.
Journal: Journal of Chromatography A - Volume 1464, 16 September 2016, Pages 55–63