کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1198808 | 1493524 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inlet backflushing device for the improvement of comprehensive two dimensional gas chromatographic separations
ترجمه فارسی عنوان
دستگاه تزریق ورودی برای بهبود جداسازی کروماتوگرافی گاز جامع دو بعدی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Comprehensive two-dimensional gas chromatography (GCÂ ÃÂ GC) is recognised as a powerful tool for the separation of complex mixtures of volatile and semi-volatile compounds. In the analysis of challenging samples containing highly concentrated, active analytes or those with complicated matrices, it is often the case that less than ideal chromatography is produced. GCÂ ÃÂ GC chromatograms of such samples typically contain broad, tailing analyte bands. This results in difficulties with quantitation and poor utilisation of the separation space. In this study we investigated the inlet and the modulator as the potential sources of these tailing bands. A simple inlet backflushing device was developed to isolate the inlet from the primary column after the injection, and a similar setup was used to isolate the modulator from the primary column. The device allowed us to divert carrier gas flow back through the inlet at a specified time after the injection, while allowing analytes to pass through the column for separation. Analytes retained within the inlet were prevented from entering the column, and were subsequently removed via the carrier gas split line. The study revealed that the inlet plays a significant role in the development of tailing chromatographic bands, while the modulator simply modulates the already elongated band. Inlet backflushing is a cheap, simple and effective tool that can be used to improve the chromatography of problematic GCÂ ÃÂ GC analyses of samples consisting of concentrated and active analytes, those derived from natural products and containing complicated matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1402, 10 July 2015, Pages 110-123
Journal: Journal of Chromatography A - Volume 1402, 10 July 2015, Pages 110-123
نویسندگان
Matthew Edwards, Tadeusz Górecki,