کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1199017 | 1493517 | 2015 | 5 صفحه PDF | دانلود رایگان |
• A approach for the study of small molecules metabolome and lipidome was developed.
• It effectively combined solid phase extraction with column-switching technology.
• It is used for the rapid and high-throughput extraction and non-targeted analysis.
• The validation results demonstrated the method is reasonable and effective.
• The approach provides a new analytical mean for biological samples.
This study combines solid phase extraction (SPE) using 96-well plates with column-switching technology to construct a rapid and high-throughput method for the simultaneous extraction and non-targeted analysis of small molecules metabolome and lipidome based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry. This study first investigated the columns and analytical conditions for small molecules metabolome and lipidome, separated by an HSS T3 and BEH C18 columns, respectively. Next, the loading capacity and actuation duration of SPE were further optimized. Subsequently, SPE and column switching were used together to rapidly and comprehensively analyze the biological samples. The experimental results showed that the new analytical procedure had good precision and maintained sample stability (RSD < 15%). The method was then satisfactorily applied to more widely analyze the small molecules metabolome and lipidome to test the throughput. The resulting method represents a new analytical approach for biological samples, and a highly useful tool for researches in metabolomics and lipidomics.
Journal: Journal of Chromatography A - Volume 1409, 28 August 2015, Pages 277–281