کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1199175 | 1493563 | 2014 | 7 صفحه PDF | دانلود رایگان |
• Several offline and online 2D RPLC methods to separate a multicomponent mixture were developed.
• A standard mixture of 12 warfarins and monohydroxylated isomers were resolved in 16.2 min.
• Online two-dimensional LC method by chiral RP-HPLC × achiral RP-UHPLC provided the fastest separation of all isomers.
Several offline and online 2D HPLC methods were investigated for the reversed phase resolution of a complex mixture of closely related warfarin and hydroxywarfarin isomers. By combining reversed phase achiral/chiral HPLC separation with UV-triggered fraction collection and subsequent chiral/achiral reversed phase HPLC analysis of collected fractions, complete resolution of all 12 components of the mixture was possible. In addition, a faster method was developed from online 2D HPLC analysis where multicomponent fractions from the first dimension are simultaneously chromatographed in the second dimension.
Journal: Journal of Chromatography A - Volume 1363, 10 October 2014, Pages 200–206