کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1199175 1493563 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigation of two-dimensional high performance liquid chromatography approaches for reversed phase resolution of warfarin and hydroxywarfarin isomers
ترجمه فارسی عنوان
بررسی روشهای دو بعدی کروماتوگرافی مایع با کارآیی بالا برای حل فاز معکوس ایزومرهای وارفارین و هیدروکسی وارفارین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Several offline and online 2D RPLC methods to separate a multicomponent mixture were developed.
• A standard mixture of 12 warfarins and monohydroxylated isomers were resolved in 16.2 min.
• Online two-dimensional LC method by chiral RP-HPLC × achiral RP-UHPLC provided the fastest separation of all isomers.

Several offline and online 2D HPLC methods were investigated for the reversed phase resolution of a complex mixture of closely related warfarin and hydroxywarfarin isomers. By combining reversed phase achiral/chiral HPLC separation with UV-triggered fraction collection and subsequent chiral/achiral reversed phase HPLC analysis of collected fractions, complete resolution of all 12 components of the mixture was possible. In addition, a faster method was developed from online 2D HPLC analysis where multicomponent fractions from the first dimension are simultaneously chromatographed in the second dimension.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1363, 10 October 2014, Pages 200–206
نویسندگان
, , ,