کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1199530 1493581 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation and evaluation of a novel monolithic column containing double octadecyl chains for reverse-phase micro high performance liquid chromatography
ترجمه فارسی عنوان
آماده سازی و ارزیابی یک ستون یکپارچه جدید حاوی زنجیره اکتادیدیل دوطبقه برای کروماتوگرافی مایع با میکرو عملکرد بالا معکوس
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A novel monolithic column containing double C18 alkyl chains was prepared.
• This monolith demonstrated good permeability, efficiency, reproducibility and selectivity.
• The monolith showed good shape selectivity towards vitamin E.
• Good separation and peak shape of intact protein was observed.

A novel reverse-phase monolithic stationary phase containing double long alkyl chains was prepared based on the thermally initiated co-polymerization of 3-methylacryloyl-3-oxapropyl-3-(N,N-dioctadecylcarbamoyl)-propionate (AOD) and ethylene glycol dimethacrylate (EDMA) in the presence of 2-methyl-1-propanol and 1,4-butanediol as the selected porogens. The polymerization was carefully optimized and good permeability, stability and column efficiency were observed for the final monolithic columns. The column also showed good long term stability and reproducibility. The methylene selectivity demonstrates typical reversed phase characteristics. The optimized poly (AOD-co-EDMA) monolith exhibited good selectivity for a range of non-polar test analytes such as PAHs, tocopherols and alkylphenones. A good separation of intact proteins was also observed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1345, 6 June 2014, Pages 174–181
نویسندگان
, , , , , , , ,