کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1199895 | 1493580 | 2014 | 9 صفحه PDF | دانلود رایگان |
• New hybrid dummy molecularly imprinted polymers.
• 3-Aminopropyltriethoxy silane-methacrylic acid as hybrid monomer.
• Miniaturized molecularly imprinted solid-phase extraction in 1.5-mL tapered centrifuge tube.
• Good recognition and selective extraction of acyclovir from urine.
The miniaturized molecularly imprinted solid-phase extraction (mini-MISPE) coupled with high-performance liquid chromatography was proposed for the determination of acyclovir in urine. 1.5-mL tapered plastic centrifuge tube filled with hybrid molecularly imprinted polymers (HMIPs) was used as the cartridge of mini-MISPE, and the HMIPs synthesized with 3-aminopropyltriethoxy silane-methacrylic acid as monomer exhibited good recognition and selectivity for acyclovir. Under the optimized condition, good linear calibration was obtained in a range of 0.5–15 μg mL−1 with the correlation coefficient of 0.9994, and the recoveries at three spiked levels were 91.6–103.3% in urine with the relative standard deviation (RSD) of ≤3.5%. Excellent intra-day and inter-day repeatability were achieved with RSD of ≤2.6% and 4.0% in three different concentrations. This method combined the advantages of HMIPs and mini-MISPE, and it could become an alternative tool for analyzing the residues of acyclovir in complex urine matrices.
Journal: Journal of Chromatography A - Volume 1346, 13 June 2014, Pages 16–24