کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1200102 1493467 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of polycyclic aromatic hydrocarbon quinones by gas chromatography-tandem mass spectrometry, following a one-pot reductive trimethylsilyl derivatization
ترجمه فارسی عنوان
تعیین همزمان هیدروکربن های چند حلقه ای معطر با استفاده از طیف سنجی جرم کروماتوگرافی-دو بعد، با استفاده از مشتقات تریتیلی سیلیل
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• 37 polycyclic aromatic hydrocarbon quinones (PAHQs) were analyzed by GC–MS/MS.
• Silylation of PAHQs was achieved by the one-pot reductive derivatization step.
• The derivatization provided characteristic fragmentations and high sensitivity.
• Novel compounds that were never reported in atmospheric samples were determined.
• The method has the potential to become a standard analytical method.

We developed a sensitive and selective method to simultaneously analyze 37 polycyclic aromatic hydrocarbon quinones (PAHQs) with GC–MS/MS and applied the method to the analysis of standard atmospheric particulate matter samples. PAHQs were reduced with zinc granules and dithiothreitol (DTT) and the reductants were immediately converted to their silylated derivatives in a test tube. Two trimethylsilyl (TMS) groups were introduced into PAHQs through the one-pot reductive TMS derivatization. The PAHQs were derivatized with a mixed silylation reagent (BSA + TMCS + TMSI; (3:2:3)), which is one of the combinations of TMS-derivatization reagents with the highest reactivity. The derivatives produced different fragmentation between o-PAHQs and p-PAHQs. Therefore, isomers that have the same molecular weight are difficult to separate on a column were separated by the selected reaction monitoring (SRM) mode using the characteristic fragmentations, allowing separation and detection of all PAHQ derivatives in less than 30 min. The instrumental detection limit (IDL) of each PAHQ was 1.2–29 fg/injection and the method quantification limit (MQL) was 0.8–78 μg/kg sample. For quantification, six deuterated PAHQs were used as internal standards to achieve high analytical precision. We applied the developed method to four standard atmospheric particulate matter samples. Results showed that out of 37 PAHQs, 33 compounds were identified and quantified. Moreover, from the 33 PAHQs, 14 were detected for the first time. Similar values were observed for the concentrations of PAHQs that have been quantified in previous reports. This method has the highest practicality in monitoring PAHQs in atmosphere, combustion exhaust gas, and toxicity evaluation. Thus, the method has the potential to become a standard analytical method for such applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1459, 12 August 2016, Pages 89–100
نویسندگان
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