کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1200302 1493618 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electromembrane extraction from aqueous samples containing polar organic solvents
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electromembrane extraction from aqueous samples containing polar organic solvents
چکیده انگلیسی


• EME can be performed from 50% methanol, ethanol, or dimethyl sulfoxide.
• EME from samples containing acetonitrile should be avoided.
• The extraction time has to be increased with organic solvents present in the sample.

Electromembrane extraction (EME) was performed from aqueous samples and from aqueous samples containing methanol, ethanol, dimethyl sulfoxide, and acetonitrile. The basic drugs pethidine, haloperidol, nortriptyline, methadone and loperamide were used as model analytes. Reversed phase (C18) HPLC with UV (235 nm) and MS detection was used for analysis of the samples. With no organic solvent in the sample, maximum recoveries were obtained after 5–10 min. The maximum recoveries ranged between 83 and 95%. With 50% (v/v) methanol, ethanol, or dimethyl sulfoxide in the sample, recoveries were comparable to those from an aqueous sample, but the time required reaching maximum recovery increased to 15–25 min. With 2-nitrophenyl octyl ether (NPOE) as the supported liquid membrane (SLM), a stable EME system was obtained for 50% (v/v) methanol, 50% (v/v) ethanol, or 75% (v/v) dimethyl sulfoxide in the sample solution. On the other hand, the EME system was unstable with acetonitrile in the sample, as this solvent partly dissolved the SLM. In addition, acetonitrile migrated through the SLM and caused a volume expansion of the acceptor solution. Other SLMs were also tested (ethyl nitrobenzene, isopropyl nitrobenzene, and dodecyl nitrobenzene), but were inferior to NPOE. As a practical example, EME on dried blood spot extracts (80% methanol) were tested, and proved highly successful. These observations showed that EME can be an effective way of preparing aqueous samples containing substantial amounts of an organic solvent.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1308, 20 September 2013, Pages 37–44
نویسندگان
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