کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1200646 1493606 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High performance liquid chromatography with evaporative light scattering detection for the characterisation of a vesicular delivery system during stability studies
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High performance liquid chromatography with evaporative light scattering detection for the characterisation of a vesicular delivery system during stability studies
چکیده انگلیسی


• Lipid analysis is important for assessment of vesicular delivery system stability.
• A NP-HPLC-ELSD method was developed for simultaneous quantification of lipids.
• Quantification of vesicle lipid constituents was possible within 10 min.
• Chemical stability of a vesicular system was successfully assessed.
• The method could be generically applied to other vesicle delivery systems.

A normal phase high performance liquid chromatography method with evaporative light scattering detection was developed for the simultaneous quantification of the lipid constituents of a non-ionic surfactant vesicle (NIV) delivery system consisting of tetra-ethylene glycol mono n-hexadecyl ether, cholesterol and dicetyl phosphate. An accelerated stability study performed at 25 °C/60% relative humidity (RH) and 40 °C/75% RH indicated that the NIV were chemically stable. Similar results were observed when stored at 4 °C for 469 days. This chromatographic method developed is a sensitive, robust and high throughput analytical technique that offers the potential for rapid quantification of lipids in liposomal and vesicular systems. The results of the chromatographic studies were supported by parallel size and zeta potential measurements.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1320, 13 December 2013, Pages 80–85
نویسندگان
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