کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1200646 | 1493606 | 2013 | 6 صفحه PDF | دانلود رایگان |

• Lipid analysis is important for assessment of vesicular delivery system stability.
• A NP-HPLC-ELSD method was developed for simultaneous quantification of lipids.
• Quantification of vesicle lipid constituents was possible within 10 min.
• Chemical stability of a vesicular system was successfully assessed.
• The method could be generically applied to other vesicle delivery systems.
A normal phase high performance liquid chromatography method with evaporative light scattering detection was developed for the simultaneous quantification of the lipid constituents of a non-ionic surfactant vesicle (NIV) delivery system consisting of tetra-ethylene glycol mono n-hexadecyl ether, cholesterol and dicetyl phosphate. An accelerated stability study performed at 25 °C/60% relative humidity (RH) and 40 °C/75% RH indicated that the NIV were chemically stable. Similar results were observed when stored at 4 °C for 469 days. This chromatographic method developed is a sensitive, robust and high throughput analytical technique that offers the potential for rapid quantification of lipids in liposomal and vesicular systems. The results of the chromatographic studies were supported by parallel size and zeta potential measurements.
Journal: Journal of Chromatography A - Volume 1320, 13 December 2013, Pages 80–85