Comparison of concentration pulse and tracer pulse chromatography: Experimental determination of eluent uptake by bridged-ethylene hybrid ultra-high performance liquid chromatography packings

► Excess volume isotherms of acetonitrile and methanol were determined on a UHPLC packing. ► The measurements were carried out by concentration and tracer pulse chromatographies. ► The results confirmed the validity of both concentration and tracer pulse chromatographies.