کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1201599 | 1493649 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evaluation of a liquid chromatography method for quality control of methylated cyclodextrins Evaluation of a liquid chromatography method for quality control of methylated cyclodextrins](/preview/png/1201599.png)
Halo C18 column (fused core particles) and Chromolith RP18 column (monolith) were evaluated in liquid chromatography in order to analyze methylated-β-cyclodextrins (Me-β-CD) with various degrees of substitution, DS such as the number of methyl groups per cyclodextrin ring. Chromolith RP18 enables a performing analysis of Me-β-CD with low DS but is not suitable for dimethyl-β-cyclodextrins (DM-β-CD). On the other hand, Halo C18 column allows an improved fingerprint of CDs having a DS from 4.9 up to a value major than 14 and avoiding the use of various chromatographic systems. Thus, liquid chromatography performed with this column and an evaporative light scattering detector can be used as a generic system for methylated CD analysis. Moreover, fused core particles of Halo C18 column enables a rapid analysis and liquid chromatography coupled with electrospray-mass spectrometry appears as a powerful tool to determine co-elution and to characterize various isomers of complex methylated-β-cyclodextrin mixtures.
► Halo C18 and Chromolith RP18 columns were evaluated to analyze methylated CDs by LC.
► Halo C18 column is suitable for rapid LC analysis of various CDs.
► LC-ELSD with this column was proposed as a generic system for CD analysis.
Journal: Journal of Chromatography A - Volume 1277, 15 February 2013, Pages 42–47