کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1201934 1493658 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A nonlinear electrophoretic model for PeakMaster: Part IV. Electromigration dispersion in systems that contain a neutral complex-forming agent and a fully charged analyte. Experimental verification
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A nonlinear electrophoretic model for PeakMaster: Part IV. Electromigration dispersion in systems that contain a neutral complex-forming agent and a fully charged analyte. Experimental verification
چکیده انگلیسی

The complete mathematical model of electromigration dispersion in systems that contain a neutral complex forming agent and a fully charged analyte was introduced in the previous part of this series of papers (Part III – Theory). The model was implemented in the newest version of our simulation program PeakMaster 5.3 that calculates the effective mobility of the analyte and its nonlinear electromigration mobility slope, SEMD, in the presence of a complex forming agent in the background electrolyte. The mathematical model was verified by both experiments and simulations, which were performed by our dynamic simulator Simul 5 Complex. Three separation systems differing in the chiral selector used (having different values for the complexation constant and the mobility of the complex) were chosen for the verification. The nonlinear electromigration mobility slope values were calculated from the simulations and the experiments that were performed at different complex forming agent concentrations. These data agree very well with those predicted by the mathematical model and provided the foundation for the discussion and explanation of the electromigration dispersion process that occurs in systems which contain a complex forming agent. The new version of PeakMaster 5.3 was shown to be a powerful tool for optimization of the separation conditions by minimizing electromigration dispersion which improves the symmetry of the analyte peaks and their resolution.


► Linearized model of electromigration of complex-forming systems is verified.
► Experimental and simulated results agree perfectly.
► EMD is in chiral separation systems mainly governed by complexation.
► Development of EMD depends on value of complexation constant and complex mobility.
► PeakMaster 5.3 is powerful tool to optimize enantiomer separation by minimizing EMD.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1267, 7 December 2012, Pages 109–115
نویسندگان
, , , , , ,