کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1202096 | 1493706 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simultaneous determination of thirty non-steroidal anti-inflammatory drug residues in swine muscle by ultra-high-performance liquid chromatography with tandem mass spectrometry Simultaneous determination of thirty non-steroidal anti-inflammatory drug residues in swine muscle by ultra-high-performance liquid chromatography with tandem mass spectrometry](/preview/png/1202096.png)
An ultra-high-performance liquid chromatography with tandem mass spectrometric detection (UHPLC–MS/MS) method was established for the simultaneous determination of residues of thirty non-steroidal anti-inflammatory drugs (NSAIDs) in swine muscle. The samples were extracted with acetonitrile and phosphoric acid. The extracts were defatted with n-hexane, and then purified by HLB solid-phase extraction cartridge. Analysis was carried out on UHPLC–ESI-MS/MS working with multiple reaction monitoring mode with polarity switching. Limits of detection were between 0.4 μg/kg and 2.0 μg/kg, and limits of quantification were between 1.0 μg/kg and 5.0 μg/kg. The recoveries of NSAIDs were between 61.7% and 125.7% at spiked levels of 1.0–500 μg/kg. The repeatability was less than 8% and the within-laboratory reproducibility was not more than 12.3%. The method was reliable, convenient and sensitive.
► An UHPLC–MS/MS method was established for the simultaneous determination of residues of thirty NSAIDs in swine muscle.
► The samples were extracted with acetonitrile and phosphoric acid, purified by HLB solid-phase extraction cartridge, and analyzed on UHPLC–ESI-MS/MS.
► The method was reliable, convenient and sensitive.
Journal: Journal of Chromatography A - Volume 1219, 6 January 2012, Pages 104–113