On-line coupled high performance liquid chromatography–gas chromatography for the analysis of contamination by mineral oil. Part 1: Method of analysis

For the analysis of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH), on-line coupled high performance liquid chromatography–gas chromatography–flame ionization detection (HPLC–GC–FID) offers important advantages: it separates MOSH and MOAH in robust manner, enables direct injection of large aliquots of raw extracts (resulting in a low detection limit), avoids contamination of the sample during preparation and is fully automated. This review starts with an overview of the technology, particularly the fundamentals of introducing large volumes of solvent into GC, and their implementation into various transfer techniques. The main part deals with the concepts of MOSH and MOAH analysis, with a thorough discussion of the choices made. It is followed by a description of the method. Finally auxiliary tools are summarized to remove interfering components, enrich the sample in case of a high fat content and obtain additional information about the MOSH and MOAH composition.

► Basics on solvent evaporation for on-line transfer of HPLC fractions.
► HPLC preseparation for MOSH and MOAH.
► HPLC–GC interfaces: conditions for HPLC–GC transfer.
► GC for mineral oil analysis.
► Auxiliary tools for mineral oil analysis.