کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1202347 1493548 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Solvent minimization in two-dimensional liquid chromatography
ترجمه فارسی عنوان
به حداقل رساندن حلال در کروماتوگرافی مایع دو بعدی
کلمات کلیدی
کروماتوگرافی مایع دو بعدی، بهینه سازی، مصرف زنده، کروماتوگرافی سبز، قدرت تفکیک، کاهش حلال
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A theoretical framework is developed for minimization of solvent consumption of 2DLC.
• The number of fractions analyzed in the second dimension affects eluent consumption.
• Effect of equilibration volume is negligible.
• General equation is derived for finding optimum number of collected fractions.

An algorithm was developed for the minimization of consumption of organic solvent in comprehensive two-dimensional liquid chromatography (2DLC). It was shown that one can reach higher peak capacities only by using more eluent. The equilibration volume of the second dimension, however, did not affect the solvent consumption significantly. Calculations confirmed that the same target peak capacity could be achieved by consuming significantly different volume of organic modifier depending on the number of fractions analyzed in the second dimension suggesting that 2D separations can be optimized for eluent consumption. It was shown that minimization of eluent usage requires the use of small and high efficient columns in the second dimension. A simple equation was derived for the calculation of the optimal number of collected fractions from the first dimension that allowed the minimization of eluent usage, cost and environmental impact of comprehensive 2DLC separations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1378, 23 January 2015, Pages 32–36
نویسندگان
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