کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1202371 | 965067 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protein UTLC-MALDI–MS using thin films of submicrometer silica particles
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Slides for ultra thin-layer chromatography (UTLC) were made by coating nonporous silica particles, chemically modified with polyacrylamide, as 15 μm films on glass or silicon. Three proteins, myoglobin, cytochrome c and lysozyme, are nearly baseline resolved by the mechanism of hydrophilic interaction chromatography. A plate height as low as 3 μm, with 3900 plates, is observed in 14 mm. Varying silica particle diameter among 900, 700 and 350 nm showed that decreasing particle diameter slightly improves resolution but slows the separation. Matrix-assisted laser desorption/ionization (MALDI)-MS of the proteins after separation is demonstrated by wicking sufficient sinapinic acid into the separation medium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 40, 7 October 2011, Pages 7196–7202
Journal: Journal of Chromatography A - Volume 1218, Issue 40, 7 October 2011, Pages 7196–7202
نویسندگان
Zhaorui Zhang, Saliya N. Ratnayaka, Mary J. Wirth,