کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1202371 965067 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein UTLC-MALDI–MS using thin films of submicrometer silica particles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Protein UTLC-MALDI–MS using thin films of submicrometer silica particles
چکیده انگلیسی

Slides for ultra thin-layer chromatography (UTLC) were made by coating nonporous silica particles, chemically modified with polyacrylamide, as 15 μm films on glass or silicon. Three proteins, myoglobin, cytochrome c and lysozyme, are nearly baseline resolved by the mechanism of hydrophilic interaction chromatography. A plate height as low as 3 μm, with 3900 plates, is observed in 14 mm. Varying silica particle diameter among 900, 700 and 350 nm showed that decreasing particle diameter slightly improves resolution but slows the separation. Matrix-assisted laser desorption/ionization (MALDI)-MS of the proteins after separation is demonstrated by wicking sufficient sinapinic acid into the separation medium.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 40, 7 October 2011, Pages 7196–7202
نویسندگان
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