کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1202634 1493690 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Taylor dispersion analysis with two detection points on a commercial capillary electrophoresis apparatus
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Taylor dispersion analysis with two detection points on a commercial capillary electrophoresis apparatus
چکیده انگلیسی

This work describes a simple technical modification for doing Taylor dispersion analysis with two UV detection points on a commercial capillary electrophoresis apparatus. So far, double UV detection was only possible using specific detectors that are external to the capillary electrophoresis apparatus. In this work, the detection interface of the capillary electrophoresis apparatus was easily modified to allow the introduction and the superposition of two capillary windows in the same interface (at the same detection point). This modification made possible the double detection of the sample zone in Taylor dispersion analysis using a loop. The peak dispersion using the modified interface was similar to that obtained on a non-modified UV interface. Diffusion coefficients (and the corresponding hydrodynamic radii) of small molecule and proteins were determined in good agreement with values of the literature and with RSD lower than 5%.


► Double UV detection on commercial CE apparatus without external detector.
► Simple technical modification on detection interface for double detection.
► Application to the sizing of protein and small molecule by Taylor dispersion analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1235, 27 April 2012, Pages 174–177
نویسندگان
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