کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1202652 | 965079 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nucleic acid affinity of clustered-charge anion exchange adsorbents: Effects of ionic strength and ligand density
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
In previous work we demonstrated the improved protein-binding capacity and selectivity of ion-exchange adsorbents displaying a “clustered” rather than random, distribution of surface charges. For example, anion-exchange adsorbents displaying 5Â mM of positive charge in the form of 1Â mM penta-argininamide show much higher affinity and capacity for alpha-lactalbumin than do adsorbents displaying the same 5Â mM total charge in the form of single dispersed argininamide charges. We also found that clustered adsorbents selectively favor proteins with inherent charge clustering. In the present work, “clustered” penta-argininamide adsorbents showed DNA binding capacity comparable to that of conventional dispersed adsorbents with 10-100-fold higher ligand density. We also observed that at moderate ionic strength the DNA affinity of all adsorbents tested increased with salt while RNA affinity decreased, so that selectivity for DNA over RNA was enhanced as salt concentration increased.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 2, 14 January 2011, Pages 258-262
Journal: Journal of Chromatography A - Volume 1218, Issue 2, 14 January 2011, Pages 258-262
نویسندگان
Wen-hsiang Chen, Joseph Y. Fu, Katerina Kourentzi, Richard C. Willson,