کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1203295 965106 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modelling oligomer formation in chromatographic separations
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Modelling oligomer formation in chromatographic separations
چکیده انگلیسی

Oligomer forms of proteins are formed by self and cross association or complex formation with ligands. Modelling studies using an ion-exchange adsorbent have demonstrated that the formation of an oligomer form of a target protein can improve a chromatographic separation because the oligomer form displaces the impurities. The results of the investigation show that the process is very robust, that the purity, the yield, and the productivity increase with increasing load and increasing salt concentration in the eluant. An impurity level less than 1 ppm is easy to achieve having a yield of 98%. A universal model for the adsorption equilibria of mono and oligomer forms of proteins on ion-exchange, hydrophobic, and bimodal adsorbents has been developed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 49, 9 December 2011, Pages 8869–8873
نویسندگان
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