کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1203328 | 1493609 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultra-high-performance liquid chromatography-tandem mass spectrometry measurement of climbazole deposition from hair care products onto artificial skin and human scalp
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ultra-high-performance liquid chromatography-tandem mass spectrometry measurement of climbazole deposition from hair care products onto artificial skin and human scalp Ultra-high-performance liquid chromatography-tandem mass spectrometry measurement of climbazole deposition from hair care products onto artificial skin and human scalp](/preview/png/1203328.png)
چکیده انگلیسی
A sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the measurement of climbazole deposition from hair care products onto artificial skin and human scalp. Deuterated climbazole was used as the internal standard. Atmospheric pressure chemical ionization (APCI) in positive mode was applied for the detection of climbazole. For quantification, multiple reaction monitoring (MRM) transition 293.0 > 69.0 was monitored for climbazole, and MRM transition 296.0 > 225.1 for the deuterated climbazole. The linear range ran from 4 to 2000 ng mLâ1. The limit of detection (LOD) and the limit of quantification (LOQ) were 1 ng mLâ1 and 4 ng mLâ1, respectively, which enabled quantification of climbazole on artificial skin and human scalp at ppb level (corresponding to 16 ng cmâ2). For the sampling of climbazole from human scalp the buffer scrub method using a surfactant-modified phosphate buffered saline (PBS) solution was selected based on a performance comparison of tape stripping, the buffer scrub method and solvent extraction in in vitro studies. Using this method, climbazole deposition in in vitro and in vivo studies was successfully quantified.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1317, 22 November 2013, Pages 155-158
Journal: Journal of Chromatography A - Volume 1317, 22 November 2013, Pages 155-158
نویسندگان
Guoqiang Chen, Michael Hoptroff, Xiaoqing Fei, Ya Su, Hans-Gerd Janssen,