کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1203364 965110 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hydrodynamic chromatography of macromolecules using polymer monolithic columns
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Hydrodynamic chromatography of macromolecules using polymer monolithic columns
چکیده انگلیسی

The selectivity window of size-based separations of macromolecules was tailored by tuning the macropore size of polymer monolithic columns. Monolithic materials with pore sizes ranging between 75 nm and 1.2 μm were prepared in situ in large I.D. columns. The dominant separation mechanism was hydrodynamic chromatography in the flow-through pores. The calibration curves for synthetic polymers matched with the elution behavior by HDC separations in packed columns with ‘analyte-to-pore’ aspect ratios (λ) up to 0.2. For large-macropore monoliths, a deviation in retention behavior was observed for small polystyrene polymers (Mr < 20 kDa), which may be explained by a combined HDC–SEC mechanism for λ < 0.02. The availability of monoliths with very narrow pore sizes allowed investigation of separations at high λ values. For high-molecular weight polymers (Mr > 300,000 Da) confined in narrow channels, the separation strongly depended on flow rate. Flow-rate dependent elution behavior was evaluated by calculation of Deborah numbers and confirmed to be outside the scope of classic shear deformation or slalom chromatography. Shear-induced forces acting on the periphery of coiled polymers in solution may be responsible for flow-rate dependent elution.


► Monoliths were prepared with macropore size tailored for hydrodynamic separation.
► Separations based on hydrodynamic chromatography were achieved.
► Selectivity of the separation was controlled by macropore size.
► Monoliths provide unique selectivity through the availability of very small pores.
► Flow-rate dependent elution was observed for large polymers in confined channels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 48, 2 December 2011, Pages 8638–8645
نویسندگان
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