Separation of poly-3-hydroxyvalerate-co-3-hydroxybutyrate through gradient polymer elution chromatography

► Poly-3-hydroxyvalerate-co-3-hydroxybutyrate copolymers polymers were separated for the first time using gradient polymer elution chromatography. ► The mechanism of the separation involved precipitation-redissolution interactions. ► Well defined fractions with varying molecular weight and % of 3-hydroxybutyrate were observed as a result of this separation.