کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1203763 | 965125 | 2011 | 7 صفحه PDF | دانلود رایگان |
A novel separation technique of complexation high-speed counter-current chromatography (HSCCC) using copper ion as a complexation agent was first developed to isolate 5-hydroxyisoflavone isomers from Belamcanda chinensis (L.) DC. According to the partition coefficient and separation factor, the two-phase solvent system composed of light petroleum–ethyl acetate–methanol–water (3:5:3:5, v/v) and copper nitrate (0.10 mol/L in the lower phase) was selected. 9.2 mg isoirigenin (1), 46.4 mg irigenin (2) and 1.2 mg 5,7,4′-trihydroxy-6,3′,5′-trimethoxyisoflavone (3) were simultaneously purified from 100 mg crude extract by HSCCC with the purity of 95.06%, 96.98% and 93.69%, respectively. As evidenced by the results of UV–Vis spectroscopy, the stoichiometries of the copper ion with the three 5-hydroxyisoflavones were all 1:1 and their chelating power was 3 > 2 > 1. Those explained the complexation HSCCC behavior. It is the first report that includes the practical application of complexation HSCCC and explanation of its chromatographic behavior.
Journal: Journal of Chromatography A - Volume 1218, Issue 14, 8 April 2011, Pages 1842–1848