Reversed-phase screening strategies for liquid chromatography on polysaccharide-derived chiral stationary phases

Immobilised polysaccharide-based chiral stationary phases (CSPs) are chromatographic materials that combine the remarkable enantioselective performance of the polysaccharide derivatives in addition to solvent versatility for enantiomeric resolution. Their behaviour under normal phase conditions and polar organic mode has been quite extensively discussed in several scientific communications. This article will focus on an approach to developing efficient chiral analytical methods with these immobilised CSPs (CHIRALPAK IA, CHIRALPAK IB and CHIRALPAK IC) by applying a limited number of mobile phases under reversed-phase conditions. The manuscript will review the development of screening strategies by liquid chromatography for the separation of enantiomers in combination with applications compatible with LC–MS. The rational combination of this technique and the different supports will allow the identification of enantiomeric resolutions in reasonable time frames and with high success rates.