کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1204312 965149 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
First approach based on direct ultrasonic assisted enzymatic digestion and capillary-high performance liquid chromatography for the peptide mapping of soybean proteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
First approach based on direct ultrasonic assisted enzymatic digestion and capillary-high performance liquid chromatography for the peptide mapping of soybean proteins
چکیده انگلیسی

This work proposes, for the first time, the use of a high intensity ultrasonic probe to accelerate the tryptic digestion of soybean proteins. Different digestion parameters were optimized: protein extracting solution, reduction, and alkylation conditions (time, concentration, and temperature), trypsin:protein ratio, and ultrasonic conditions (sonication amplitude and time). Separation of peptide profiles was carried out by capillary-HPLC. The effect of the variation of chromatographic conditions (elution gradient, column temperature, and injection volume) on peptide separation was also studied using two capillary-HPLC columns with different column diameters and particle sizes. Moreover, samples were focused at the top of the column in order to obtain an increasing sensitivity without loss of efficiency. This method was successfully applied to the profiling of soybean peptides from transgenic and non-transgenic soybeans and from different pigmented beans commercialized as soybeans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1217, Issue 42, 15 October 2010, Pages 6443–6448
نویسندگان
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